Coding

Part:BBa_K357001:Design

Designed by: Anish Kapadia   Group: iGEM10_Toronto   (2010-06-23)

muconate and chloromuconate cycloisomerase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 799
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was optimized for E. coli. Additionally, a BgIII restriction site was removed from bp 531 and an NgoMIV restriction site was removed from bp246. Finally, a PstI restriction site was present at bp 124, which then disappeared after the sequence optimization.

Source

AUTHORS Nelson,K., Paulsen,I., Weinel,C., Dodson,R., Hilbert,H., Fouts,D., 

          Gill,S., Pop,M., Martins Dos Santos,V., Holmes,M., Brinkac,L.,

          Beanan,M., DeBoy,R., Daugherty,S., Kolonay,J., Madupu,R.,

          Nelson,W., White,O., Peterson,J., Khouri,H., Hance,I., Lee,P.,

          Holtzapple,E., Scanlan,D., Tran,K., Moazzez,A., Utterback,T.,

          Rizzo,M., Lee,K., Kosack,D., Moestl,D., Wedler,H., Lauber,J.,

          Hoheisel,J., Straetz,M., Heim,S., Kiewitz,C., Eisen,J., Timmis,K.,

          Duesterhoft,A., Tummler,B. and Fraser,C.

TITLE     Complete genome sequence and comparative analysis of the

          metabolically versatile Pseudomonas putida KT2440

JOURNAL   Environ. Microbiol. 4 (12), 799-808 (2002)


References